Cloning and further sequence analysis of the ORF3 gene of wild- and attenuated-type porcine epidemic diarrhea viruses
Identifieur interne : 002C65 ( Main/Exploration ); précédent : 002C64; suivant : 002C66Cloning and further sequence analysis of the ORF3 gene of wild- and attenuated-type porcine epidemic diarrhea viruses
Auteurs : Seong-Jun Park [Corée du Sud] ; Hyoung-Joon Moon [Corée du Sud] ; Yuzi Luo [Corée du Sud] ; Hye-Kwon Kim [Corée du Sud] ; Eun-Mi Kim [Corée du Sud] ; Jeong-Sun Yang [Corée du Sud] ; Dae-Sub Song [Corée du Sud] ; Bo-Kyu Kang [Corée du Sud] ; Chul-Seung Lee [Corée du Sud] ; Bong-Kyun Park [Corée du Sud]Source :
- Virus Genes [ 0920-8569 ] ; 2008-02-01.
Descripteurs français
- Wicri :
- topic : Clonage.
English descriptors
Abstract
Abstract: The open reading frame (ORF3) genes of the parent DR13, attenuated DR13, KPED-9, P-5V, and 12 field samples were cloned and sequenced to further explore the functions of wild- and attenuated-type porcine epidemic diarrhea viruses (PEDVs). Sequencing revealed that wild-type PEDVs ORF3 genes had a single ORF of 675 nucleotides encoding a protein of 224 amino acids with a predicted M r of 25.1–25.3 kDa. Attenuated-type PEDVs ORF3 genes had a single ORF of 624 nucleotides encoding a protein of 207 amino acids with a predicted M r of 23.4 kDa. The coding region of the ORF3 gene of attenuated-type PEDVs including attenuated DR13, KPED-9, and P-5V had 51 nucleotide deletions that were not found in the ORF3 genes of wild-type PEDVs including CV777, Br1/87, LZC, parent DR13, and 12 field samples. In addition, attenuated-type PEDVs have previously been found to exhibit reduced pathogenicity in pigs. Therefore, 51 nucleotide deletions appear to be meaningful and may be significant for PEDV pathogenicity, because they lead to changes in the predicted amino acid sequences of attenuated-type PEDVs. Reverse transcriptase-polymerase chain reaction (RT-PCR) on the partial ORF3 gene including 51 nucleotide deletions revealed that all PEDVs fell into two types, wild- and attenuated-type PEDVs. Wild-type PEDVs containing parent DR13 and 12 field samples had RT-PCR products of 245 bp in size, while attenuated-type PEDVs containing PEDV vaccine strains (attenuated DR13, KPED-9, P-5V) had products of 194 bp. In addition, all PEDV vaccine strains were used as live virus vaccine, because they previously exhibited a reduced pathogenicity in pigs. Therefore, large deletion region, which is comprise 17 amino acid deletions caused by 51 nucleotide deletions and is seen in all PED live vaccine strains, may be important site for PEDV pathogenicity, and we can use it for differentiation of wild- and attenuated-type PEDVs.
Url:
DOI: 10.1007/s11262-007-0164-2
Affiliations:
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Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Kim, Eun Mi" sort="Kim, Eun Mi" uniqKey="Kim E" first="Eun-Mi" last="Kim">Eun-Mi Kim</name><affiliation wicri:level="4"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, 599 Gwanangno, 151-742, Gwanak-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Yang, Jeong Sun" sort="Yang, Jeong Sun" uniqKey="Yang J" first="Jeong-Sun" last="Yang">Jeong-Sun Yang</name><affiliation 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Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Corée du Sud</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Genes</title><title level="j" type="abbrev">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2008-02-01">2008-02-01</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="95">95</biblScope><biblScope unit="page" to="104">104</biblScope></imprint><idno type="ISSN">0920-8569</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0920-8569</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning</term><term>ORF3 gene</term><term>Pathogenicity</term><term>Porcine epidemic diarrhea virus</term><term>RT-PCR</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Clonage</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The open reading frame (ORF3) genes of the parent DR13, attenuated DR13, KPED-9, P-5V, and 12 field samples were cloned and sequenced to further explore the functions of wild- and attenuated-type porcine epidemic diarrhea viruses (PEDVs). Sequencing revealed that wild-type PEDVs ORF3 genes had a single ORF of 675 nucleotides encoding a protein of 224 amino acids with a predicted M r of 25.1–25.3 kDa. Attenuated-type PEDVs ORF3 genes had a single ORF of 624 nucleotides encoding a protein of 207 amino acids with a predicted M r of 23.4 kDa. The coding region of the ORF3 gene of attenuated-type PEDVs including attenuated DR13, KPED-9, and P-5V had 51 nucleotide deletions that were not found in the ORF3 genes of wild-type PEDVs including CV777, Br1/87, LZC, parent DR13, and 12 field samples. In addition, attenuated-type PEDVs have previously been found to exhibit reduced pathogenicity in pigs. Therefore, 51 nucleotide deletions appear to be meaningful and may be significant for PEDV pathogenicity, because they lead to changes in the predicted amino acid sequences of attenuated-type PEDVs. Reverse transcriptase-polymerase chain reaction (RT-PCR) on the partial ORF3 gene including 51 nucleotide deletions revealed that all PEDVs fell into two types, wild- and attenuated-type PEDVs. Wild-type PEDVs containing parent DR13 and 12 field samples had RT-PCR products of 245 bp in size, while attenuated-type PEDVs containing PEDV vaccine strains (attenuated DR13, KPED-9, P-5V) had products of 194 bp. In addition, all PEDV vaccine strains were used as live virus vaccine, because they previously exhibited a reduced pathogenicity in pigs. Therefore, large deletion region, which is comprise 17 amino acid deletions caused by 51 nucleotide deletions and is seen in all PED live vaccine strains, may be important site for PEDV pathogenicity, and we can use it for differentiation of wild- and attenuated-type PEDVs.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Park, Seong Jun" sort="Park, Seong Jun" uniqKey="Park S" first="Seong-Jun" last="Park">Seong-Jun Park</name></region><name sortKey="Kang, Bo Kyu" sort="Kang, Bo Kyu" uniqKey="Kang B" first="Bo-Kyu" last="Kang">Bo-Kyu Kang</name><name sortKey="Kim, Eun Mi" sort="Kim, Eun Mi" uniqKey="Kim E" first="Eun-Mi" last="Kim">Eun-Mi Kim</name><name sortKey="Kim, Hye Kwon" sort="Kim, Hye Kwon" uniqKey="Kim H" first="Hye-Kwon" last="Kim">Hye-Kwon Kim</name><name sortKey="Lee, Chul Seung" sort="Lee, Chul Seung" uniqKey="Lee C" first="Chul-Seung" last="Lee">Chul-Seung Lee</name><name sortKey="Luo, Yuzi" sort="Luo, Yuzi" uniqKey="Luo Y" first="Yuzi" last="Luo">Yuzi Luo</name><name sortKey="Moon, Hyoung Joon" sort="Moon, Hyoung Joon" uniqKey="Moon H" first="Hyoung-Joon" last="Moon">Hyoung-Joon Moon</name><name sortKey="Park, Bong Kyun" sort="Park, Bong Kyun" uniqKey="Park B" first="Bong-Kyun" last="Park">Bong-Kyun Park</name><name sortKey="Park, Bong Kyun" sort="Park, Bong Kyun" uniqKey="Park B" first="Bong-Kyun" last="Park">Bong-Kyun Park</name><name sortKey="Song, Dae Sub" sort="Song, Dae Sub" uniqKey="Song D" first="Dae-Sub" last="Song">Dae-Sub Song</name><name sortKey="Yang, Jeong Sun" sort="Yang, Jeong Sun" uniqKey="Yang J" first="Jeong-Sun" last="Yang">Jeong-Sun Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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